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Research articles

ScienceAsia 44(2018): 179-186 |doi: 10.2306/scienceasia1513-1874.2018.44.179


Determination of antioxidant activity of gum arabic: An exudation from two different locations


Mohamed E.S. Mirghania,b,*, Ahmed A.M. Elnoura,c, N.A. Kabbashia, Md Z. Alama, Khalid Hamid Musad, Amina Abdullahd

 
ABSTRACT:     Gum arabic (GA) is the main product of acacia trees. As a raw and commercial samples, GA was extracted with methanol and analysed to measure the antioxidant activity using five methods: 2,2-diphenyl-1-picrylhydrazyl (DPPH), Folin-Ciocalteu indexes (FCI), which indicate total phenolic compounds (TPC), oxygen radical absorbance capacity (ORAC), ferric reducing antioxidant power (FRAP), and cupric reducing antioxidant capacity (CUPRAC). This study used antioxidant assays to detect TPC and selected appropriate and inexpensive methods to determine the antioxidant capacity of GA samples. The results reveal that the FCI, ORAC, and CUPRAC are correlated highly with FRAP. Person's correlation coefficient (r) values are 0.98, 0.93, and 0.99, respectively, based on the sample size of (n=8). This means that the TPC of GA is highly correlated with their antioxidant activities that are measured by these three methods. Hence the FCI, ORAC, and the CUPRAC methods are more effective and simpler. They had similar predictive power to the FRAP of GA antioxidant activity. Consequently, GA is generally recognized as being slightly acidic which may have been obtained from appropriate methods of the antioxidant capacity detection. This acidity is due to the electronic transfer mechanism based on the selection of the working pH.

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a Bioenvironmental Engineering Research Centre, Biotechnology Engineering, Faculty of Engineering, International Islamic University, Malaysia, PO Box 10, Gombak 50728 Kuala Lumpur, Malaysia
b International Institute for Halal Research and Training,  International Islamic University Malaysia, PO Box 10, Gombak 50728 Kuala Lumpur, Malaysia
c Department of Biochemistry & Gum Processing, Gum Arabic Research Centre, University of Kordofan, Box 160 Elobied, Sudan
d School of Chemical Sciences & Food Technology, Faculty of Science & Technology, 43600 UKM, Bangi Selangor, Malaysia

* Corresponding author, E-mail: elwathig@iium.edu.my

Received 1 Jun 2017, Accepted 22 Apr 2018