| Home  | About ScienceAsia  | Publication charge  | Advertise with us  | Subscription for printed version  | Contact us  
Editorial Board
Journal Policy
Instructions for Authors
Online submission
Author Login
Reviewer Login
Volume 45 Number 4
Volume 45 Number 3
Volume 45 Number 2
Volume 45 Number 1
Volume 44 Number 6
Volume 44 Number 5
Earlier issues
Volume 45 Number 2 Volume 45 Number 3 Volume 45 Number 4

previous article next article 1

Research articles

ScienceAsia 45(2019): 236-244 |doi: 10.2306/scienceasia1513-1874.2019.45.236


Antiplatelet and fibrinogenolytic activities of a purified mucus protein from Eudrilus eugeniae (African night crawler)


Kittidet Prem-U-domkita, Montamas Suntravatb, Sara E. Lucenab, Elda E. Sánchezb,d, Anchanee Kuberaa,c,*

 
ABSTRACT:     Proteins from the earthworm mucus have been revealed for their various biological activities. In this study, a 38-kDa protein, earthworm antiplatelet agent (EAPA) was purified from the earthworm Eudrilus eugeniae (African night crawler) mucus and examined for its antiplatelet and fibrinogenolytic activities. EAPA was found to possess fibrinogenolytic activity on α- and β-chains of fibrinogen. It inhibited ADP-, collagen-, and ristocetin-induced platelet aggregation. This protein also had antiplatelet function on clot retraction, which was evaluated by a Sonoclot Analyser. N-terminal amino acid sequences of EAPA shared 65% identity with lysenin, an earthworm haemolytic protein. EAPA may have potential in the development of therapeutic tools in the treatment of thrombotic diseases.

Download PDF

46 Downloads 101 Views


a Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900 Thailand
b National Natural Toxins Research Center (NNTRC), Texas A&M University-Kingsville, Kingsville, TX 78363 USA
c Center for Advanced Studies in Tropical Natural Resources, Kasetsart University, Bangkok 10900 Thailand
d Department of Chemistry, Texas A&M University-Kingsville, Kingsville, TX 78363 USA

* Corresponding author, E-mail: fsciacs@ku.ac.th

Received 20 Aug 2018, Accepted 29 Jun 2019