Research articles
ScienceAsia 45 (2019): 229-235 |doi:
10.2306/scienceasia1513-1874.2019.45.229
Supplementation of sugarcane molasses for
maximization of ethanol production by
Saccharomyces cerevisiae using response surface
method
Pongpannee Phomikheta, Wanlapa Lorliamb, Suthep Thaniyavarna, Somboon Tanasupawatc,
Ancharida Savarajaraa,?
ABSTRACT: Dilute sugarcane molasses containing 19.2% fermentable sugars was supplemented with (NH4)2SO4,
KH
2PO4, and MgSO4 - 7 H2O to maximize the ethanol production by Saccharomyces cerevisiae TISTR 5596 using a
central composite design of response surface method. The maximum ethanol (87.28 g/l) was produced when the
dilute molasses was supplemented with 417 mg/l NH+ 4 , 1.88 g/l PO3- 4 and 5 mg/l Mg2+. The Ca2+ contaminated in the
molasses was proposed as an indicative index for the requirement of phosphate and Mg2+ supplementation to maximize
the ethanol production.
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| a |
Department of Microbiology, Faculty of Science, Chulalongkorn University, Bangkok 10330 Thailand |
| b |
Department of Microbiology, Faculty of Science, Srinakharinwirot University, Bangkok 10110 Thailand |
| c |
Department of Biochemistry and Microbiology, Faculty of Pharmaceutical Science,
Chulalongkorn University, Bangkok 10330 Thailand |
* Corresponding author, E-mail: sanchari@chula.ac.th
Received 25 Mar 2018, Accepted 4 Jun 2019
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