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Preventive effects of polyphenol-rich perilla leaves on oxidative stress and haemolysis

WachirapornÿTipsuwan, WittayaÿChaiwangyen^*

 
ABSTRACT:     We investigated the effect of perilla leaf extract (PLE) containing polyphenols on various biological functions including antioxidant activity, iron chelation, and red blood cell haemolysis protection. The total phenolic and flavonoid content were determined by colorimetric assay and HPLC analysis. The antioxidant activity was determined by DPPH and ABTS assay. The iron chelating effect was examined by the colorimetric method. Free radical scavenging activity was determined by haemoglobin-induced lipid peroxidation in a linoleic acid model system. Reactive oxygen species (ROS) inhibition was investigated in peripheral blood mononuclear cells. The antihaemolytic activity by AAPH-induced free radicals. The results revealed that PLE contained high levels of total phenolic compounds and total flavonoids (mainly rosmarinic acid). The IC₅₀ values of PLE by DPPH and ABTS radical scavenging assay were 34.3±3.4 and 9.69±0.14ÿµg/ml, respectively. The iron-binding effect of PLE was exhibited by complex formation with Fe³⁺-NTA. The iron-chelating activity displayed a moderate IC₅₀ value of 10.1±0.6ÿmg/ml. The PLE concentration of 200ÿµg/ml, significantly inhibited intracellular ROS 45%. The scavenging activity of PLE in haemoglobin-induced lipid peroxidation resulted in 27% inhibition. PLE also prevented AAPH-induced RBC haemolysis. In conclusion, PLE containing phenolic compounds plays a vital role in free radical scavenging and iron-chelating activities and subsequently prevents lipid peroxidation resulting from RBC haemolysis due to oxidative stress. Perilla products may be developed as health supplements, which could probably be used in prophylaxis and treatment of non-communicable diseases.

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* Corresponding author, E-mail: wittaya.ch@up.ac.th

Received 11 May 2018, Accepted 16 Jul 2018