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ScienceAsia 37 (2011): 170-173 |doi: 10.2306/scienceasia1513-1874.2011.37.170

Single-nucleotide primer extension assay of mtDNA to authenticate cattle and buffalo meat

Rohita Guptaa,*, D.N. Rankb, C.G. Joshib

ABSTRACT:     A reliable method for identifying and differentiating cattle and water buffalo meat by fluorescence-based single-nucleotide primer extension assay is presented. The method used genotyping (single nucleotide primer extension) of the cyt b gene region of mitochondrial DNA extracted from meat samples. Meat samples were subjected to different levels of autolysis to simulate various meat processing technologies. The assay indicates absolute specificity and a high sensitivity, even to low DNA concentrations of 0.01 pg (1%) of adulteration in cattle-buffalo meat mixture, and can be applied with equal efficiency to fresh, cooked, or putrefied meat.

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a Indian Veterinary Research Institute, Izatnagar, Uttar Pradesh 243122, India
b College of Veterinary Science, Anand Agricultural University, Anand- 388001, India

* Corresponding author, E-mail: rohitagupta2001@gmail.com

Received 6 Nov 2010, Accepted 18 May 2011