Research articles
ScienceAsia (): 383-387 |doi:
10.2306/scienceasia1513-1874...383
A novel role for ribonuclease 9 in suppressing sperm
capacitation and acrosome reaction
Jie Liua, Yuanyuan Houb, Lei Gonga, Yuanyuan Gub, Lijun Duana, Yuxiao Tangc, Wenting Wanga,*
ABSTRACT: Ribonuclease 9 (RNase 9) is a member of the ribonuclease A superfamily, which is mainly expressed in
the epididymis. However, it has no ribonucleolytic activity and its function remains unclear. Immunofluorescence
experiments showed that the localization of RNase 9 protein on the sperm surface has moved from the neck to the
acrosome cap during capacitation and acrosome reaction. Chlortetracycline staining was used to assay the role of
RNase 9 on capacitation and acrosome reaction. The spontaneous acrosome reaction induced by progesterone could be
suppressed by RNase 9 protein and the acrosome reaction ratio was 6.5?1.2%. Furthermore, exposure of spermatozoa
to RNase 9 protein reduced the level of cyclic AMP significantly during the acrosome reaction. Taken together, we
confirmed that RNase 9 protein could impair sperm function, including capacitation and acrosome reaction, and had
potential role in male reproductive toxicity
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| a |
Biochip Laboratory, Yantai Yuhuangding Hospital affiliated to Qingdao University, Yantai 264000 China |
| b |
Obstetrics Department, Yantai Yuhuangding Hospital affiliated to Qingdao University, Yantai 264000
China |
| c |
Endocrine Department, Yantai Yuhuangding Hospital affiliated to Qingdao Universit, Yantai 264000
China |
* Corresponding author, E-mail: Endocrine Department, Yantai Yuhuangding Hospital affiliated to Qingdao Universit, Yantai 264000Ch
Received 28 Apr 2018, Accepted 17 Dec 2018
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