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Research articles

ScienceAsia 45(2019): 109-115 |doi: 10.2306/scienceasia1513-1874.2019.45.109


Anti-Helicobacter pylori, anti-inflammatory and antioxidant evaluation of crude extracts from Amomum krervanh fruits


Bhanuz Dechayonta,*, Pathompong Phuakleea, Jitpisute Chunthorng-Orna, Saovapak Poomirata, Onmanee Prajuabjindaa, Ratha-korn Vilaichoneb, Arunporn Itharata,c

 
ABSTRACT:     The aim of the present study was to determine the antibacterial properties against Helicobacter pylori and the anti-inflammatory and antioxidant activities of crude A. krervanh extracts. The minimum inhibitory concentrations (MICs) of ethanolic and aqueous extracts were measured against standard strain H. pylori standard (ATCC 43504) and six clinical isolates by disc diffusion and agar dilution. Anti-inflammatory activities were evaluated by measuring the inhibition of nitric oxide (NO) production in LPS-activated RAW 264.7 macrophages using the Griess assay and the ELISA measurement of proinflammatory cytokines, tumour necrosis factor ? (TNF-?), and interleukin 6 (IL-6). Their cytotoxic activities against macrophages were determined by the MTT assay. ABTS and DPPH scavenging assays measured the antioxidant activities. Only the ethanolic extract showed antibacterial activity with mean inhibition zones of 9.0?19.3 mm for a corresponding MIC of 250 ?g/ml; the clarithromycin MICs ranged from 0.5?1 ?g/ml. This extract also inhibited production of NO (mean IC50 98.99?0.39 ?g/ml) and IL-6 (mean IC50 18.68?2.16 ?g/ml) and exhibited moderate antioxidant activity using DPPH and ABTS scavenging assays: mean IC50s 95.92?1.26 and 90.35?2.25 ?g/ml, respectively. Thus ethanolic extract showed a greater spectrum of activity that could be exploited further as adjunct treatments of H. pylori related gastrointestinal disease.

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a Department of Applied Thai Traditional Medicine, Faculty of Medicine, Thammasat University, Pathum Thani, Thailand
b Gastroenterology Unit, Department of Medicine, Thammasat University, Pathum Thani, Thailand
c Center of Excellence in Applied Thai Traditional Medicine Research, Thammasat University, Pathum Thani, Thailand

* Corresponding author, E-mail: Nuzz@hotmail.com

Received 2 Apr 2018, Accepted 5 Apr 2019