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Research articles

ScienceAsia 42(2016): 375-381 |doi: 10.2306/scienceasia1513-1874.2016.42.375


Cytotoxicity and genotoxicity of Allamanda and Plumeria species


Arunrat Chaveeracha,b, Tawatchai Taneeb,c, Natcha Patarapadungkitb,d, Peerapat Khamwachirapithakb, Runglawan Sudmoonb,e,*

 
ABSTRACT:     Species of Allamanda and Plumeria have been used as medicinal plants for a long time. This study investigates the cytotoxicity and genotoxicity of their extracts on lymphocytes and HeLa cells using cell viability tests and comet assay. A. cathartica, A. violacea, P. obtusa, and P. rubra were collected and hexane-extracted. The maximum concentrations of crude extracts were 315, 625, 357, and 303 mg/ml, respectively. For cytotoxicity on lymphocytes treated with the maximum final concentration of the four extracts, the cell viabilities were 79%, 96%, 70%, and 60%, respectively. In HeLa cells, the IC50 was 13.5, 3.75, 21.5, and 14.5 mg/ml, respectively. These extracts showed significant genotoxicity (p<0.01) on both lymphocytes and HeLa cells. These findings suggest that Allamanda and Plumeria should not be used as medicinal plants.

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a Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand
b Genetics and Environmental Toxicology Research Group, Khon Kaen University, Khon Kaen 40002 Thailand
c Faculty of Environment and Resource Studies, Mahasarakham University, Maha Sarakham 44150 Thailand
d Department of Pathology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002 Thailand
e Faculty of Law, Khon Kaen University, Khon Kaen 40002 Thailand

* Corresponding author, E-mail: rsudmoon@yahoo.com

Received 27 May 2017, Accepted 0 0000