Research articles
ScienceAsia (): 75-81 |doi:
10.2306/scienceasia1513-1874...075
Antioxidant activity and cytotoxicity against cholangiocarcinoma of carbazoles and coumarins from Clausena harmandiana
Uraiwan Songsianga, Tula Thongthooma, Pornsilp Zeekpudsab, Veerapol Kukongviriyapanb, Chantana Boonyaratc, Jinda Wangboonskulc, Chavi Yenjaia,*
ABSTRACT: Nine carbazoles and three coumarins were isolated from C. harmandiana. Crude extracts and all isolates were evaluated for antioxidant activity using 2,2-diphenyl-1-picrylhydrazyl (DPPH) and lipid peroxidation assay and cytotoxicity against cholangiocarcinoma, KKU-OCA17 and KKU-214 cell lines. 7-Hydroxyheptaphylline and nordentatin showed strong antioxidant activity on lipid peroxidation with IC50 values of 2.95 and 2.90 µM respectively, while the activity on a DPPH assay showed IC50 values of 56.82 and 29.3 µM, respectively. The two compounds exhibited strong cytotoxicity against KKU-OCA17 (IC50=88.7 and 46.1 µM, respectively) and KKU-214 (IC50=43.7 and 39.1 µM, respectively) cell lines. The results show convincingly that these compounds may be the promising leads for the development of cytotoxic agents.
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| a |
Natural Products Research Unit, Department of Chemistry and Centre for Innovation in Chemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002 Thailand |
| b |
Department of Pharmacology, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002 Thailand |
| c |
Faculty of Pharmaceutical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand |
* Corresponding author, E-mail: chayen@kku.ac.th
Received 14 Oct 2011, Accepted 19 Jan 2012
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