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Research articles

ScienceAsia 37 (2011): 205-213 |doi: 10.2306/scienceasia1513-1874.2011.37.205

Analysis of the immediate early-1 gene sequence of Thai Bombyx mori nucleopolyhedrovirus and its usefulness for identifying the subgroups of group II nucleopolyhedroviruses

Banthari Chotimanothuma, Thanaporn Pinpartb, Lertluk Ngernsiric,*

ABSTRACT:     The immediate early 1 (ie-1) gene is essential for DNA replication of nucleopolyhedrosis virus. Here, the gene ie-1 from nucleopolyhedrovirus (Nanglai-BmNPV) infecting a local silkworm (Bombyx mori), Nanglai strain, was cloned and sequenced. The gene contained a 1755 bp open reading frame encoding a deduced protein of 584 amino acids, molecular weight 66.9 kDa, and isoelectric point 5.79. The nucleotide and amino acid sequences of Nanglai-BmNPV ie-1 gene showed, respectively, 98 and 97% identity to those of the BmNPV ie-1 genes. The identity of the Nanglai-BmNPV IE-1 deduced amino acid sequence with IE-1 protein sequences of other 37 known Lepidopteran NPVs varied from 20% up to 97%. The phylogenic tree established with the IE-1 protein sequences of the Lepidopteran NPVs showed similar topology to those previously reported using highly conserved genes. Interestingly, the tree obtained with IE-1 clearly shows that the Lepidopteran NPVs group II clade can be subdivided into three subgroups.

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a Bioscience Program, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand
b National Laboratory Animal Center, Mahidol University, Nakhon Pathom 73170, Thailand
c Department of Genetics, Faculty of Science, Kasetsart University, Bangkok 10900, Thailand

* Corresponding author, E-mail: khawlln@yahoo.com

Received 3 Feb 2011, Accepted 11 Apr 2011