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Research articles

ScienceAsia 37 (2011): 219-224 |doi: 10.2306/scienceasia1513-1874.2011.37.219

Application of fractional factorial design to optimize extraction method of artemisinin from Artemisia annua

Munawar Khalila,*, Latifah K. Darusmanb,c, Utami D. Syafitrid

ABSTRACT:     Fractional factorial design was applied to optimize the extraction process of artemisinin from Artemisia annua L. Three different techniques i.e., Soxhlet, maceration, and microwave-assisted extraction were optimized to determine the optimum extraction conditions. Thin layer chromatography and high performance liquid chromatography were used to determine the yield of artemisinin in the sample. The optimum condition was obtained by immersing 0.5 g of sample in 3 ml of chloroform for 2 min. The amount of artemisinin extracted from the sample was 47.9±0.6 × 10−3% w/w. In addition, the predicted values calculated by the model were in good agreement with the experimental values.

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a Department of Chemical Engineering, Faculty of Engineering, University of Malaya, Kuala Lumpur 50603, Malaysia
b Biopharmaca Research Center, Bogor Agricultural University, Jl. Taman Kencana No. 3, Bogor 16151, Indonesia
c Department of Chemistry, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia
d Department of Statistic, Faculty of Mathematics and Natural Sciences, Bogor Agricultural University, Bogor, Indonesia

* Corresponding author, E-mail: khalilmunawar@gmail.com

Received 9 Dec 2010, Accepted 11 May 2011