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Research articles

ScienceAsia 35 (2009): 156-160 |doi: 10.2306/scienceasia1513-1874.2009.35.156

Effects of amyloid-β peptide on glutamine transporter mRNA expression and cell viability in cultured rat cortical cells

Doungjai Buntupa, Anek Chayasadomb, Rudee Suraritc, Nuanchan Jutapakdeegula, Wipawan Thangnipona,*

ABSTRACT:     Alzheimer's disease is a major neurodegenerative disorder in which there is an overproduction and accumulation of amyloid-β (Aβ) peptides. During the initial stages of the disease, glutamate receptors are dysregulated by Aβ accumulation resulting in the disruption of glutamatergic synaptic transmission. We used rat cortical cell cultures to examine the effects of Aβ(25–35)-induced neurotoxicity on glutamine transporters involved in the glutamate cycle. In primary mixed cell cultures prepared from cerebral cortex, incubation with 10 µM Aβ(25–35) for 12 h, but not for 24 h, markedly suppressed system A transporter 1 (SAT1) mRNA expression. On the other hand, Aβ(25–35) had no effect on SAT1 mRNA level in neuronal cell cultures. Treatment of both types of cell cultures with Aβ(25–35) resulted in a significant decrease in cell survival in a concentration and time-dependent manner, as determined by MTT assay. These results indicated that Aβ may impair neuronal function and transmitter synthesis and perhaps reduce excitotoxicity through a reduction in neuronal glutamine uptake.

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a Neuro-Behavioural Biology Centre, Institute of Science and Technology for Research and Development, Mahidol University, Nakorn Pathom 73170, Thailand
b Department of Periodontology, Faculty of Dentistry, Khon Kaen University, Khon Kaen 40002, Thailand
c Department of Physiology and Biochemistry, Faculty of Dentistry, Mahidol University, Bangkok 10400, Thailand

* Corresponding author, E-mail: grwtn@mahidol.ac.th

Received 11 Nov 2008, Accepted 27 May 2009