Research articles
ScienceAsia 49 (2023):ID 864-872 |doi:
10.2306/scienceasia1513-1874.2023.094
Effects of growth regulators on production of bioactive
compounds from Clinacanthus nutans (Burm.f.) Lindau
"Phaya Yo" culture in vitro
Monthar Wongmaneeroj, Surak Jamjumrus, Rattana Agarum, Siriphan Sukkhaeng*, Somnuk Promdang,
Rongrong Homhual
ABSTRACT: Clinacanthus nutans is a valuable plant that is harvested for medicinal purposes in Southeast Asia. However,
the production of herbal medicines needs raw, uncontaminated materials with a consistent quantity of bioactive
compounds. Therefore, in vitro culture is of interest for the production of secondary metabolites. The present study
applied plant growth regulators to stimulate bioactive compounds from multiple shoots of Clinacanthus nutans. Nodal
explants were studied for multiple shoot induction using different types of plant growth regulators: thidiazuron (TDZ)
alone or in combination with 1-naphthaleneacetic acid (NAA). The obtained calli or multiple shoots were examined
for bioactive compounds (total phenolic, flavonols/flavones, phytosterols and antioxidant activity). The results showed
that TDZ at concentrations of 0.5?1.0 mg/l either alone or in combination with NAA at concentrations of 0.01?0.1 mg/l
stimulated a higher total phenolic content (including antioxidant activity) than the control. At such concentrations,
TDZ and NAA were more likely to stimulate lupeol production in the tissue and its content was higher than in the leaves
from the pot-grown plants. The present result revealed suitable growth regulator factors for stimulating the production
of bioactive compounds, such as total phenolic compounds, antioxidants and phytosterols and especially lupeol, which
were found in the tissues of C. nutans at a level 98.25-folds higher than in the leaves from the pot-grown plants. This
technique could support the production of raw materials containing known quantities of various bioactive compounds
at the laboratory level and be used for further research on the creation of quantitative bioactive compounds in C. nutans
herbs for commercial production.
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Central Laboratory and Greenhouse Complex, Research and Academic Service Center,
Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kampheang Sean Campus,
Nakhon Pathom Thailand 73140 |
* Corresponding author, E-mail: fagrsps@ku.ac.th
Received 7 Jun 2023, Accepted 24 Sep 2023
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