Research articles
ScienceAsia 48 (2022):ID 726-733 |doi:
10.2306/scienceasia1513-1874.2022.103
pPeOp from Omphalia lapidescens Schroet suppresses the
proliferation of cervical cancer HeLa cells via JAK/STAT3
signaling pathway
Yan Chen, Zhenjie Fu, Yuqin Xu, Yitao Chen*, Peilei Pan*
ABSTRACT: pPeOp is a protein extracted from the sclerotium of Omphalia lapidescens. Several studies have shown its
anti-carcinogenic effects. However, its functions in cervical cancer and the underlying cellular mechanisms are relatively
unknown. Here, we examined the effect and molecular mechanism of pPeOp on HeLa cells. The MTS assay and EdU
assay showed that pPeOp decreased Hela cell viability and proliferation in a dose-dependent manner. Besides, the
quantitative real-time PCR (qRT-PCR) and western blot analysis showed that the mRNA levels of JAK1, JAK2, GP130,
and STAT3 and protein levels of STAT3 and p-STAT3 were significantly decreased, whereas the mRNA expression levels
of SOCS1 and SOCS3 were significantly elevated in HeLa cells. Furthermore, Interleukin-6 (IL-6) and NSC74859 were
used as the agonist and inhibitor of the JAK/STAT3 signaling pathway, respectively. The flow cytometry and MTS assay
showed that the antitumor effect was increased when pPeOp was co-treated with IL-6, while decreased with inhibitor
treatment. These results demonstrate that pPeOp could effectively inhibit the proliferation and viability of cervical
cancer cells by regulating JAK/STAT3 signaling pathway, suggesting that pPeOp may serve as a novel therapeutic agent
for cervical cancer
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School of Life Sciences, Zhejiang Chinese Medical University, Hangzhou 310053 China |
* Corresponding author, E-mail: cytworld@163.com, panplei@163.com
Received 19 Sep 2021, Accepted 5 May 2022
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