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Research articles

ScienceAsia 48 (2022): 32-36 |doi: 10.2306/scienceasia1513-1874.2022.007

Effects of octacosanol on HMG-CoA reductase and cyclooxygenase-2 activities in the HT-29 human colorectal cancer cell line

Suphaket Saenthaweesuka, Atcharaporn Thaeomorb, Pornrut Rabintossaporna, Jarinyaporn Naowaboota, Nuntiya Somparna,*

ABSTRACT:     Octacosanol (OCT) is a major component of policosanol which has been reported to possess anti-inflammatory and lipid-lowering effects. Therefore, it was our interest to evaluate the effects of OCT on HMG-CoA reductase (HMGR) and cyclooxygenase-2 (COX-2) activities in a human colorectal cancer cell line (HT-29). Our results demonstrate that 100 µM OCT decreased viability of HT-29 cells as analyzed by sulforhodamine B colorimetric assay with more pronounced effects seen in cells treated with atorvastatin (AST) or celecoxib (CLX), the inhibitors to HMGR and COX-2, respectively. Additionally, the activity of HMGR was found to be inhibited in cells treated with OCT, while COX-2 activity was unaffected; these effects were also more pronounced in AST- and CLX-treated cells. In cells treated with OCT, a significant decrease in HMGR protein expression was observed, but there was no alteration in COX-2 protein expression as determined by Western blot. Taken together, our results show that OCT inhibited HT-29 cell growth and that this effect might be attributed to the reduction of HMGR protein expression and activity.

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a Preclinical Science, Faculty of Medicine, Thammasat University 12120 Thailand
b School of Preclinic, Institute of Science, Suranaree University of Technology, Nakhon Ratchasima 30000 Thailand

* Corresponding author, E-mail: nuntiya_tom@hotmail.com

Received 24 Mar 2021, Accepted 22 Jul 2021