Volume  Number

previous article

next article

Extraction and evaluation of biological activities of a polysaccharide from Allium mongolicum Regel

Ruiping Hu^a,?, Songyao Xu^a,?, Changjin Ao^b, Yanhua Ma^a, Hashen Bao^a, Shumei Ye^a, Jitao Sun^a, Hong Yuan^a,*, Huiting Xue^a,*, Chunli Ma^a,*

 
ABSTRACT:      Allium mongolicum Regel polysaccharide (AMRP) acts as a main active substance of A. mongolicum Regel, but its structure and function are not fully understood. In this study, the AMRP fraction, AMRP-2 consisting of GalA, Rha, Glc, and Gal, was isolated and characterized. The antibacterial activity of AMRP-2 evaluated with the cup-plate method had a weak inhibitory effect against Staphylococcus aureus, Staphylococcus albus, Pseudomonas aeruginosa, and Proteus vulgaris, but Escherichia coli and Shigella dysenteriae were relatively sensitive to AMRP-2. Furthermore, the immunoregulatory effects of AMRP-2 were evaluated both in vitro and in vivo. The results showed that after intragastric administration of AMRP-2, the proliferation of splenic T cells and the phagocytosis of peritoneal macrophages in mice were significantly enhanced, and the interleukin-2 (IL-2) and tumour necrosis factors ? (TNF-?) levels in serum also increased sharply compared to the control group. These in vivo results were consistent with those of the in vitro study, i.e., splenic T cells proliferated dramatically after AMRP-2 treatment, and the secretion of IL-2 from mouse splenic T cells and TNF-? from peritoneal macrophages increased significantly. These findings indicated that AMRP-2 is an important immune modulator that has potential as an immunotherapy drug.

Download PDF

136 Downloads 1852 Views

* Corresponding author, E-mail: 20080292@immu.edu.cn, huiting410@hotmail.com, ma200614127@126.com

Received 22 Jan 2021, Accepted 10 Dec 2021