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Volume 47 Number 5 Volume 47 Number 6 Volume 48 Number 1

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Research articles

ScienceAsia 47 (2021): 698-706 |doi: 10.2306/scienceasia1513-1874.2021.100

Effects of extraction methods on the flavonoid and phenolic contents and anti-aging properties of Rhyncholaeliocattleya Haw Yuan Beauty extracts

Rittipun Rungruanga,*, Tasanee Panichakula, Wittawat Rattanathavorna, Nattapon Kaisangsrib, Orapin Kerdchoechuenc, Natta Laohakunjitc, Louis Kuoping Chaod

ABSTRACT:     Rhyncholaeliocattleya Haw Yuan Beauty (Rlc. Haw Yuan Beauty) is a traditional orchid in tropical and subtropical Asian regions, which has attracted attention for its fragrant flowers with diverse colors. This study aimed to investigate the effects of different extraction methods on the phenolic profile, total flavonoid and cyanidin-3-O-glucoside contents, and anti-aging properties of Rlc. Haw Yuan Beauty crude extracts. The crude extracts were obtained using solvent, ultrasonic, and supercritical carbon dioxide fluid extraction methods. The major phenolic compounds including gallic, chlorogenic, and caffeic acids were present in all the extracts. The extract obtained with solvent extraction showed the highest total flavonoid content, whereas that obtained with supercritical fluid extraction yielded the highest cyanidin-3-O-glucoside content. Additionally, the different extraction methods had a significant influence on the antioxidant, anti-elastase, and anti-collagenase activities (p < 0.05) of Rlc. Haw Yuan Beauty extracts. The crude extracts derived from the ultrasonic and supercritical carbon dioxide fluid extraction methods were not cytotoxic to human skin fibroblasts at any of the tested concentrations (31500 µg/ml). Thus, the crude extracts from Rlc. Haw Yuan Beauty could be a safe and active ingredient in the production of skincare products in the cosmetic industry for human use.

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a Department of Cosmetic Science, Faculty of Science and Technology, Suan Dusit University, Bangkok 10700 Thailand
b Pilot Plant Development and Training Institute, King Mongkut?s University of Technology Thonburi (Bangkhuntein Campus), Bangkok 10150 Thailand
c Division of Biochemical Technology, School of Bioresources and Technology, King Mongkut?s University of Technology Thonburi (Bangkhuntein Campus), Bangkok 10150 Thailand
d Department of Cosmeceutics, China Medical University, Taichung 40402 Taiwan

* Corresponding author, E-mail: rittipun_run@dusit.ac.th

Received 25 Nov 2020, Accepted 24 Aug 2021