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Research articles

ScienceAsia 47 (2021): 287-292 |doi: 10.2306/scienceasia1513-1874.2021.030

Serum carbonic anhydrase combined with adiponectin as biomarkers of insulin resistance

Kanyanath Piumngama,*, Pilaiwan Siriprungponga, Sittiruk Roytrakulb

ABSTRACT:     Insulin resistance is the main cause of type 2 diabetes. The hyperinsulinemic-euglycemic clamp is a gold standard method for determination of the insulin resistance. However, the test cannot directly measure the insulin resistance, and it is time consuming, costly, labor-intensive and requires an experienced operator. This study aims to develop the protein marker for insulin resistance. Two serum proteins, i.e. carbonic anhydrase and adiponectin were determined by using sandwich enzyme-linked immunosorbent assay (ELISA) in 3 groups of volunteersí sera: healthy group (n = 35), insulin resistance group (n = 32) and diabetic group (n = 32). We explored the associations between these proteins and the insulin resistance. Adiponectin (cutoff ≤ 4.73 µg/ml, area under the curve [AUC] = 0.644 [0.51, 0.777]) (p < 0.05) and carbonic anhydrase (cutoff ≥ 47 pg/ml, AUC = 0.65 [0.518, 0.783] (p < 0.050) have the potential as the biomarkers for diabetes. When used together, adiponectin at cutoff level ≤ 4.96 µg/ml and carbonic anhydrase at cutoff level ≥ 36 pg/ml have the potential as the biomarkers for insulin resistance (AUC = 0.664 [0.532, 0.796]) (p < 0.05). These serum protein levels will be a useful tool to diagnose the insulin resistance in human and to guide the right direction of treatment and the behavior change accordingly.

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a Department of Medical Technology, Faculty of Allied Health Sciences, Thammasat University, Pathum Thani 12121 Thailand
b Genome Institute, National Center for Genetic Engineering and Biotechnology, Pathum Thani 12121 Thailand

* Corresponding author, E-mail: kanyanath.p@allied.tu.ac.th

Received 27 Oct 2020, Accepted 24 Jan 2021