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Volume 46 Number 6

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Research articles

ScienceAsia 46 (2020): 665-670 |doi: 10.2306/scienceasia1513-1874.2020.087


Evaluation of 3 (4, 5 dimethyl-2-thiazolyl)-2, 5 diphenyl tetrazolium bromide method for rapid detection of vancomycin-resistant enterococci


Nucharat Panthasria, Prajuab Chaimaneeb, Suthida Kenpromb, Patcharaporn Tippayawatc, Nicha Charoensric, Aroonwadee Chanawongc, Ratree Tavichakorntrakoolc, Aroonlug Lulitanondc,*

 
ABSTRACT:     Vancomycin-resistant enterococci (VRE) have become a global problem of hospital-associated infections. A rapid VRE detection and early isolation of the positive cases are essential for prevention and control of their transmission in hospitals. The aim of this study was to develop and evaluate a simple and rapid method for detection of VRE by using 3 (4, 5 dimethyl-2-thiazolyl)-2, 5 diphenyl tetrazolium bromide (MTT) colorimetric method compared with standard broth microdilution (BMD) and Epsilometer test (Etest). A total of 85 Enterococcus isolates (75 vancomycinnon-susceptible, 2 vancomycin-intermediate and 8 vancomycin-susceptible) were studied. The minimum inhibitory concentrations (MICs) of vancomycin for all isolates were tested by BMD, MTT and Etest methods. The MTT method gave results within 9 h with good category agreement, essential agreement, very major, major, and minor errors (100, 92.9, 0, 0 and 7.1%, respectively), which were comparable to those of the Etest (97.6, 95.3, 2.4, 0 and 4.7%, respectively). The Cohen?s kappa coefficient (?) of the MTT method compared with the BMD showed perfect agreement (1.000) (p < 0.001). The MTT method is simple and rapid for early VRE detection to support prevention and control the spread of the enterococci in hospitals.

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a The 8th Regional Medical Science Center, Udon Thani 41330 Thailand
b Clinical Microbiology Unit, Srinagarind Hospital, Khon Kaen University, Khon Kaen 40002 Thailand
c Centre for Research and Development of Medical Diagnostic Laboratories (CMDL), Faculty of Associated Medical Sciences, Khon Kaen University, Khon Kaen 40002 Thailand

* Corresponding author, E-mail: arolul@kku.ac.th

Received 15 Jul 2020, Accepted 14 Oct 2020