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Research articles

ScienceAsia 47 (2021): 30-39 |doi: 10.2306/scienceasia1513-1874.2021.003

In vitro colchicine-induced polyploids from different explant segments of Bacopa monnieri

Kawee Sujipulia,b, Phithak Inthimab,c, Nonglak Yimtragoold, Netnaphis Warnnissorne, Prateep Warnnissornf, Surisak Prasarnpung,h,*

ABSTRACT:     Bacopa monnieri (Bmo) is a highly valued medicinal plant, widely used for several pharmacological products in Thailand. The diploid progenitor of Bmo grown in the wild as a natural source has low biomass and bacoside contents. Fifteen treatment combinations between three Bmo explant types (apical shoot, node and leaf segments) and five various colchicine concentrations (0.00, 0.05, 0.075, 0.1, and 0.5% w/v) were determined for a number of polyploid inductions, including bacoside contents and gene expression. Results showed that low concentration (0.05%) of colchicine was more effective in inducing multiple shoots (17.88 regenerants per explant) and tetraploid plantlets (15%) from leaf segments than other treatment combinations. Tetraploid clone (no.4x-3) showed the highest BmoOSC gene expression, bacoside-A3 (4.276 ± 0.019 mg/g dry weight) and bacoside-C contents (5.040 ± 0.078 mg/g dry weight) compared to diploid progenitors and mixoploid plants. These findings indicated that colchicine-induced tetraploids could have beneficial uses for genetic improvements to increase the medicinal value of local Bmo herbal medicine powder production in Thailand.

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a Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000 Thailand
b Center of Excellence in Research for Agricultural Biotechnology, Naresuan University, Phitsanulok 65000 Thailand
c Plant Tissue Culture Research Unit, Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000 Thailand
d Department of Biology, Faculty of Science, Naresuan University, Phitsanulok 65000 Thailand
e Department of Biochemistry, Faculty of Medical Science, Naresuan University, Phitsanulok 65000 Thailand
f Department of Internal Medicine, Faculty of Medicine, Naresuan University, Phitsanulok 65000 Thailand
g School of Medical Sciences, University of Phayao, Phayao 56000 Thailand
h Center of Excellence on Biodiversity, Ministry of Higher Education, Science, Research and Innovation, Bangkok 10330 Thailand

* Corresponding author, E-mail: surisak.pr@up.ac.th

Received 25 May 2020, Accepted 26 Oct 2020