Research articles
ScienceAsia 46 (2020): 713-723 |doi:
10.2306/scienceasia1513-1874.2020.084
The protective effect of Thai Herbal Sahatsatara
formula against white matter injury after chronic
cerebral hypoperfusion in middle-aged rats
Saruda Kuraeiada, Anchalee Vattarakorna, Onusa Thamsermsangb, Pravit Akarasereenontb,c,
Sompol Tapechuma, Kanokwan Tilokskulchaia, Narawut Pakaprota,*
ABSTRACT: Permanent bilateral common carotid artery occlusion (2VO) in rodents induces chronic cerebral hypoperfusion, mimicking vascular dementia in elderly people. It causes inflammation and oxidative stress, leading to
neuronal loss in the hippocampus, white matter injuries and impairments of spatial learning and memory. Thai
Herbal Sahatsatara formula (STF) has anti-inflammatory and antioxidant actions which might protect neurons and
white matter. This experiment was to investigate the possible protective effects of STF. Twenty-eight middle-aged
male Wistar rats (12 months old) were divided into 4 groups: Sham+sterile water (SW), 2VO+SW, 2VO+STF300 and
2VO+STF1000. All animals daily received either SW, STF 300 mg/kg or STF 1000 mg/kg orally after 2VO induction
until the end of the experiment. Spatial learning and memory test were examined after 2VO induction for 60 days
whereas the numbers of hippocampal neurons and white matter changes in the corpus callosum were investigated after
the end of the behavioral test. Chronic cerebral hypoperfusion significantly caused spatial learning and memory deficits
and white matter injuries in the corpus callosum while the numbers of hippocampal neurons were not significantly
affected. STF (1000 mg/kg) attenuated the impairments of spatial learning and learning flexibility and white matter
injuries. This is the first study to demonstrate the ability of Sahatsatara formula to attenuate spatial learning and
learning flexibility impairments after chronic cerebral hypoperfusion by protecting the white matter in the corpus
callosum. The results support the potential application of Sahatsatara formula against chronic cerebral hypoperfusion.
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| a |
Department of Physiology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Thailand |
| b |
Center of Applied Thai Traditional Medicine, Faculty of Medicine Siriraj Hospital, Mahidol University,
Bangkok 10700 Thailand |
| c |
Department of Pharmacology, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700
Thailand |
* Corresponding author, E-mail: narawut.pak@mahidol.ac.th
Received 9 Apr 2020, Accepted 7 Aug 2020
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