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Research articles

ScienceAsia 46 (2020): 392-396 |doi: 10.2306/scienceasia1513-1874.2020.044


A phospholipase A2 of Daboia siamensis venom suppressed expression of genes and proteins in MAPK pathway


Suchitra Khunsap*, Orawan Khow, Sunutcha Suntrarachun, Supatsorn Boonchang

 
ABSTRACT:     High mortality rate of Melanoma is due to poor response to radiotherapy and chemotherapy. A phospholipase A2 (PLA2) of Daboia siamensis venom has been reported to inhibit melanoma cells. The aim of this study is to evaluate the effects of dssPLA2 on gene and protein expression of MAPKs (Mitrogen-activated protien kinases) in melanoma cells. The mRNA and protein were determined by quantitative real-time PCR and western blotting to detect the expression levels of MEK1, MEK2, BRAF V600E and ERK1/2. Results indicated that 0.25 µg/ml of dssPLA2 reduced the mRNA expression level of these genes in a time dependent manner. Interestingly, at 48 h, the mRNA expression level of all genes was extremely decreased compared with untreated cells in the same condition. Whereas, the protein level of MEK1, MEK2, BRAF V600E and ERK1/2 were clearly reduced after dssPLA2 incubation. Our results show that dssPLA2 is a strong inhibitor to MAPK genes at 48 h.

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a College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004 China
b Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang, Jinhua 321004 China
c Xingzhi College, Zhejiang Normal University, Jinhua 321004 China
d Jinhua Polylechnic, Jinhua 321004 China

* Corresponding author, E-mail: sthaithumnas@gmail.com

Received 17 Sep 2019, Accepted 26 May 2020