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Research articles

ScienceAsia 46 (2020): 30-36 |doi: 10.2306/scienceasia1513-1874.2020.007

A novel silkworm pupae carboxymethyl chitosan inhibits mouse L929 fibroblast proliferation

Lin Zhu, Zuo-Qing Fan, Xin-Qin Shi, Na Wang, Ying-Ying Bo, Hong-En Guo*

ABSTRACT:     Postoperative intestinal adhesions are common and serious complications after surgery that can cause pain and potential mortality. Our previous study confirmed that silkworm pupae carboxymethyl chitosan (SP-carboxymethyl chitosan) reduced postoperative adhesion in vivo. Here, we elucidated the inhibitory effects of SP-carboxymethyl chitosan on mouse L929 fibroblasts. Cells were exposed to SP-carboxymethyl chitosan for 72 h, then the inhibitory effects were assessed via transforming growth factor-β1 (TGF-β1)/Smads, plasminogen activator inhibitor 1 (PAI-1), and tissue-type plasminogen activator (t-PA) signaling. The results showed that SP-carboxymethyl chitosan suppressed cell hyperplasia and significantly attenuated the gene and protein expressions of the TGF-β1/Smads signaling pathways. We also confirmed that t-PA/PAI-1 greatly increased for all SP-carboxymethyl chitosan-treated groups compared to the control. These findings suggest that SP-carboxymethyl chitosan may affect L929 cell proliferation through the TGF-β1/Smads signaling pathway to prevent adhesion after an operation.

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a Shandong Institute of Sericulture, Yantai 264001 China

* Corresponding author, E-mail: guohongen@shandong.cn

Received 9 Jul 2019, Accepted 4 Jan 2020