Analysis of nutritional composition, antioxidant activity and callus induction of Oryza sativa cultivars Khumthan and Norprae

Natthiya Chaichana

ABSTRACT: The purpose of this study was to investigate the nutritional composition, antioxidant activity and callus induction of Oryza sativa cultivars Khumthan and Norprae, which are the local rice cultivars in Chiang Saen district, Chiang Rai province, Thailand. Nutritional information was examined by AOAC method. It was found that fat, proteins, carbohydrates, crude fibre and ash composition of both rice cultivars were varied in the range of 2.56–2.91, 6.53–9.09, 71.87–76.36, 1.60–2.03, and 1.14–2.10%, respectively. Furthermore, twelve kinds of minerals including N, P, K, Ca, Mg, Mn, S, B, Na, Fe, Zn, and Cu were also detected in both cultivars. For antioxidant analysis, O. sativa cultivar Norprae had antioxidant activity of 19.39 µmol TE/g, while cultivar Khumthan had the lower value of antioxidant activity (2.4 µmol TE/g). Callus induction of both cultivars was examined. The seeds were surface sterilized with 15% Clorox for 10 min. The sterilized seed embryos were transferred to MS (Murashige and Skoog, 1962) medium supplemented with 0, 0.5, 1.0, 2.0 mg/l 2,4-D (2,4-dichlorophenoxyacetic acid) or kinetin. The result revealed that MS medium supplemented with 0.5 mg/l 2,4-D provided the highest callus induction of cultivars Khumthan and Norprae with 1.344 and 1.385 g fresh weight, respectively.

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^a	Science Program, Faculty of Education, Chiang Rai Rajabhat University, Chiang Rai 57100 Thailand

* Corresponding author, E-mail: natthiya.cha@crru.ac.th

Received 2 Apr 2019, Accepted 10 Oct 2019