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Research articles

ScienceAsia 45 (2019): 581-588 |doi: 10.2306/scienceasia1513-1874.2019.45.581


Effects on detoxification enzymes of Helicoverpa armigera (Lepidoptera: Noctuidae) infected by Beauveria bassiana spores and detection of its infection by PCR


Wanida Petlamula,*, Sawai Boukaewa, Caroline Hauxwellb, Poonsuk Prasertsanc

 
ABSTRACT:     This research aimed to investigate virulence of Beauveria bassiana36 and its pathogenicity on the infected larvae of Helicoverpa armigera. The virulence was measured by LD50 values and pathogenicity was monitored from detoxification enzymatic changes and pathogenic detection using polymerase chain reaction (PCR). LD50 of B. bassiana against H. armigera larvae was highly effective at a very low dose of 7.2 × 108 spores/ml. Phenoloxidase (PO), glutathione S-transferase (GST), esterase (EST), and glucose oxidase (GOX) activities were found from the hemolymph of the infected H. armigera larvae. PO and GST activities from the infected larval sample increased from 53.72 ± 1.10 to 79.03 ± 1.22 U/mg protein and from 3.81 ± 0.17 to 5.77 ± 0.09 U/mg protein, respectively, from 048 h and then decreased from 61.75 ± 1.12 to 37.46 ± 1.06 U/mg protein and from 4.45 ± 0.09 to 2.61 ± 0.12 U/mg protein from 72120 h, respectively. EST and GOX activities from the infected larval sample increased from 10.24 ± 0.44 to 21.84 ± 0.64 U/mg protein and from 3.38 ± 0.30 to 10.76 ± 0.34 U/mg protein, respectively, from 072 h, but decreased slightly from 21.72 ± 0.76 to 16.00 ± 0.89 U/mg protein and from 4.33 ± 0.55 to 4.02 ± 0.41 U/mg protein from 96120 h, respectively. The presence of B. bassiana in the infected larvae was detected at post-inoculation using PCR with the specific primers (P1P3). B. bassiana was successfully and rapidly detected and identified within 72120 h with molecular techniques.

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a College of Innovation and Management, Songkhla Rajabhat University, Songkhla, Thailand
b Earth Environmental and Biological Sciences, Bioscience, Faculty of Science and Engineering, Queensland University of Technology, Brisbane, Queensland, Australia
c Research and Development Office, Prince of Songkla University, Hat Yai, Songkhla, Thailand

* Corresponding author, E-mail: wanida.pe@skru.ac.th

Received 6 Nov 2018, Accepted 29 Nov 2019