ScienceAsia 33 (2007): 283-292 |doi: 10.2306/scienceasia1513-1874.2007.33.283

Antioxidant and Cytoprotective Activities of Methanolic Extract from Garcinia mangostana Hulls

Nuttavut Kosem^a, Youn-Hee Han^b and Primchanien Moongkarndi^a*

 
ABSTRACT:     Garcinia mangostana has been documented in Thai traditional medicine in various aspects. Although many benefits have been claimed, few scientific reports are available in the literature. In the present study, the methanolic extract from G. mangostana hulls (GME) was assessed for antioxidant and cytoprotective activities. The results showed that GME contained phenolic compounds and possessed reducing power as well as Fe²⁺ chelating activity. The antioxidant properties were determined by scavenging DPPH, nitric oxide and lipid radicals in dose-dependent manners. In particular, the powerful scavenging activities were found against hydroxyl and superoxide radicals when investigated using ESR spectrometry. GME also enhanced the cell survival by decreasing the oxidative damage in ECV304 endothelial cells after H₂O₂ exposure. These data indicated that GME played a pivotal role on the antioxidant and chemopreventive activities via a reducing mechanism and inhibition of intracellular oxidative stress, respectively.

KEYWORDS: Antioxidant activity, cytoprotective activity, electron spin resonance (ESR) spectrometry, Garcinia mangostana.

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^a Department of Microbiology, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
^b Department of Civil & Environmental Engineering, Faculty of Engineering, Tohoku-Gakuin University, Sendai, 981-3122, Japan.

* Corresponding author, E-mail: pypmk@mahidol.ac.th

Received 31 Jan 2007, Accepted 25 Apr 2007