Application of AFLP Technique to the Study of Calving Interval Trait of Thai Swamp Buffalo (Bubalus bubalis)

Benchamart Moolmuang^a, Supajit Sraphet^a, Ancharlie Na-Chiangmai^b, Duncan R. Smith^a, Sakol Panyim^a,c and Kanokporn Triwitayakorn^a*

ABSTRACT: This study aimed to develop genetic markers specific for the calving interval (CI) trait of the domestic Thai buffalo (Bubalus bubalis) using the technique of Amplified Fragment Length Polymorphism (AFLP). Using two groups of buffalo with extreme CI phenotypes, several AFLP markers were identified. The DNA sequences of all identified polymorphic AFLP fragments were determined by DNA sequencing and analyzed with BLAST searches. Interestingly, the analysis showed that the sequence of the AFE7M24-2 marker has a high similarity with the prostaglandin f2α receptor gene, while the sequences of the AFE16M25-2 and AFE4M28-3 markers have high similarity with the follicle stimulating hormone receptor gene of sheep (Ovis aries).

KEYWORDS: AFLP, SCAR, Thai swamp buffalo, calving interval trait.

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^a Institute of Molecular Biology and Genetics, Mahidol University, Salaya, Nakhon Pathom 73170, Thailand.
^b Department of Livestock Development, Phayathai Rd., Bangkok, 10400, Thailand. c Department of Biochemistry, Faculty of Science, Mahidol University, Phayathai, Bangkok, 10400, Thailand.

* Corresponding author, E-mail: mbktw@mahidol.ac.th

Received 17 May 2006, Accepted 8 Nov 2006

Application of AFLP Technique to the Study of Calving Interval Trait of Thai Swamp Buffalo (Bubalus bubalis)

Benchamart Moolmuanga, Supajit Srapheta, Ancharlie Na-Chiangmaib, Duncan R. Smitha, Sakol Panyima,c and Kanokporn Triwitayakorna*

Benchamart Moolmuang^a, Supajit Sraphet^a, Ancharlie Na-Chiangmai^b, Duncan R. Smith^a, Sakol Panyim^a,c and Kanokporn Triwitayakorn^a*