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Short Report
ScienceAsia 32 (2006): 201-204 |doi: 10.2306/scienceasia1513-1874.2006.32.201
Production of Polyclonal Antibodies against
Recombinant VP26 Structural Protein of White Spot
Syndrome Virus (WSSV)
Parin Chaivisuthangkura,a Phiromsak Phattanapaijitkul,b Nitaya Thammapalerd,b Sombat
Rukpratanporn,c Siwaporn Longyant,a Weerawan Sithigorngula and Paisarn Sithigorngula,*
ABSTRACT: A portion of the VP26 gene (VP26F109) encoding a structural protein of white spot syndrome virus
was cloned into an expression vector and transformed into E. coli. The objective was to produce a truncated
VP26 structural protein lacking the N-terminal transmembrane region. After induction, the recombinant
protein rVP26F109 was produced, purified by SDS-PAGE and used to immunize Swiss mice for polyclonal
antibody production. The mouse anti VP26 antiserum demonstrated specific immunoreactivity to viral
antigen in white spot syndrome virus (WSSV) infected Penaeus monodon, as verified by immunohistochemistry
and western blot. This constitutes the first step in producing monoclonal antibodies against rVP26F109 that
can be combined with anti-VP28 monoclonal antibodies to enhance the sensitivity in WSSV immunological
assays.
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a Department of Biology, Faculty of Science, Srinakharinwirot University, Bangkok 10110, Thailand.
b Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University,
Bangkok 10400, Thailand.
c Center of Excellence for Marine Biotechnology at Chulalongkorn University, National Center for
Genetic Engineering and Biotechnology (BIOTEC), Bangkok 10330, Thailand.
* Corresponding author, E-mail: paisarn@swu.ac.th
Received 24 Aug 2005,
Accepted 30 Nov 2005
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