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Research Article
ScienceAsia 32 (2006): 017-020 |doi: 10.2306/scienceasia1513-1874.2006.32.017
Localization of Plaunotol in the Leaf of
Croton stellatopilosus Ohba
Worapan Sitthithaworn,a Buppachart Potduangb and Wanchai De-Eknamkulc*
ABSTRACT: Plaunotol, a well-known protective factor-enhancing agent isolated from the leaves of Croton
stellatopilosus Ohba (Thai name : Plau-Noi, known previously as Croton sublyratus Kurz), was shown to be
accumulated in the chloroplasts of palisade mesophyll cells. This was confirmed by both detection of
plaunotol in specific leaf tissues and examination of electron microscopic pictures of subcellular organelles of
the plaunotol-containing tissue. The detection of plaunotol by a TLC-densitometric technique showed the
presence of 95% of the plaunotol in the palisade cells and only about 5% in the spongy mesophyll cells.
Inside the palisade mesophyll cells, the electron micrographs showed the presence of numerous oil globules
in the chloroplasts. Since plaunotol is an acylic diterpenoid (C-20) and is nonpolar, it was suggested that the
observed oil globules are sites of plaunotol accumulation.
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Department of Chemical Engineering, Faculty of Engineering, Kasetsart University, 50 Paholyothin Road,
Jatujak, Bangkok, 10900, Thailand.
a Department of Pharmaceutical Chemistry and Pharmacognosy, Faculty of Pharmacy, Srinakharinwirot
University, Nakornnayok 26120, Thailand.
b Department of Pharmaceuticals and Natural Products, Thailand Institute of Scientific and Technological
Research, Bangkok 10900, Thailand.
c Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Chulalongkorn University,
Bangkok 10330, Thailand
* Corresponding author, E-mail: Wanchai.D@Chula.ac.th
Received 27 Sep 2005,
Accepted 1 Nov 2005
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