ScienceAsia 31 (2005): 403-408 |doi: 10.2306/scienceasia1513-1874.2005.31.403

Protein profiles in response to salt stress in leaf sheaths of rice seedlings

Kanlaya Kong-ngern,^a* Sakda Daduang,^b Chaisiri Wongkham,^c Sumontip Bunnag,^a Manit Kosittrakun^a and Piyada Theerakulpisut^a

 
ABSTRACT: Salt-induced changes in protein synthesis in leaf sheaths of the Thai rice cv. Leaung Anan were investigated using one-dimensional SDS-PAGE and two-dimensional PAGE (2DE). In SDS-PAGE, two protein bands (22 and 31 kDa) whose expression was specifically increased under salt stress were identified. Enhanced expression of these protein bands was consistently observed in plants grown under greenhouse conditions saline during different time of the year. In 2DE, at least thirteen different proteins were induced and ten different proteins were decreased by salt. Western blot analysis of the 31 kDa of rice seedlings of three cultivars: salt-tolerant Pokkali, moderately tolerant Leuang Anan, and salt-sensitive KDML 105, showed different expression patterns. The protein was strongly induced by salinity in leaf sheaths of treated tolerant Pokkali and Leuang Anan, compared to control plants. In contrast, its level in the sensitive KDML 105 remained unchanged with salt treatment. Moreover, rice cv. Leaung Anan showed higher expression of the 31 kDa protein than Pokkali. These results suggest that the 31 kDa polypeptide is a salt-induced protein in rice leaf sheaths.

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a Applied Taxonomic Research Center, Department of Biology, Khon Kaen University, Khon Kaen 40002,
Thailand.
b Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand.
c Department of Biochemistry, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand.

* Corresponding author, E-mail: kkanla@kku.ac.th

Received 23 Mar 2005, Accepted 6 Jul 2005