Multiple Genes Determining Brown Planthopper (Nilaparvata lugens Stal) Resistance in Backcross Introgressed Lines of Thai Jasmine Rice ‘KDML105’

Jirapong Jairin^a, Theerayut Toojinda^b,*, Somvong Tragoonrung^b, Sathit Tayapat^c and Apichart Vanavichit^d

ABSTRACT: The brown planthopper (BPH), Nilaparvata lugens Stål, is one of the most serious insect pests in rice production worldwide. The BPH resistance genes in an indica cultivar ‘Abhaya’ were studied using 400 BC₄F₂ and F₃ backcross introgressed lines of KDML105 derived from a cross between Abhaya and KDML105. The BC₄F₂ plants were used for DNA analysis. Two local BPH populations collected from central and northeastern Thailand were used to evaluate the BPH resistance in the 400 BC₄F₃. Through bulked segregant analysis, four AFLP fragments were co-segregated with the BPH resistance. Linkage analysis revealed that these fragments were localized on rice chromosomes 6, 10 and 12. These map locations were in the same genomic regions where major BPH resistance genes or quantitative resistance loci were previously reported. These results indicated that multiple BPH resistance genes play major roles for BPH resistance in Abhaya. These genes may be a useful BPH resistance resource for rice breeding programs.

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a Ubon Ratchathani Rice Research Center, Muang, Ubon Ratchathani, 34000, Thailand.
b Rice Gene Discovery Unit, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.
c Pathum Thani Rice Research Center, Pathum Thani 12110, Thailand.
d Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand.

* Corresponding author, E-mail: theerayut@dna.kps.ku.ac.th

Received 26 Aug 2004, Accepted 3 Mar 2005