ABSTRACT: Microsatellites represent an abundant source for genetic markers in eukaryotic genomes. We developed
microsatellite or simple sequence length polymorphism (SSLP) markers for the black tiger shrimp genetic
improvement programs. Construction of a genetic linkage map was initiated for
Penaeus monodon using 57
SSLPs, EST and SCAR markers with 76 individuals of an F1 inter-cross family. Segregation markers were
scored and analyzed with the JoinMap 2.0 program. A total of 50 markers, confirmed with expected Mendelian
segregation ratios of 1:1 or 1:1:1:1 (
-test at the
= 0.01 level), were grouped with a LOD score of 5.0.
Results showed that 27 loci formed 9 linkage groups and there were 23 unlinked loci. The largest group had
7 markers spanning 29.5 cM. The average distance between markers was 3.8 cM. This linkage map covered
a total genetic distance of 103.6 cM.
a Interdisciplinary Graduate Program of Genetic Engineering, Faculty of Graduate School, Kasetsart
University, Bangkok 10900, Thailand.
b DNA Technology Laboratory, National Center for Genetic Engineering and Biotechnology, Kasetsart
University, Kamphaengsaen Campus, Nakorn Pathom 73140, Thailand.
c Marine Science Department, Fisheries Faculty, Kasetsart University, Bangkok 10900, Thailand.
d Rice Genes Discovery Unit, National Center for Genetic Engineering and Biotechnology, Kasetsart
University, Kamphaengsaen Campus, Nakorn Pathom 73140, Thailand.
* Corresponding author, E-mail:
suwitwuth@hotmail.com
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