Comparison of Polyclonal Anti-CD147 Antibody Production Using DNA Based and Phage-Displayed CD147 Immunizations

ABSTRACT: Immunization with two different immunogens, i.e., plasmid encoding CD147 (pCDM8-CD147) and phage-displayed CD147 were compared for producing polyclonal antibodies. Two mice were injected with pCDM8-CD147 or phage-displayed CD147 at bi-weekly intervals and antibody responses were determined by indirect ELISA and immunofluorescence. The anti-CD147 antibodies could be detected in the immunized sera after inoculating with either pCDM8-CD147 or phage-displayed CD147. However, the antibody response induced by phage-displayed CD147 was much higher than that by pCDM8-CD147. These results suggested the possibility of using the phage display technique for preparing hyperimmune serum when purified protein antigens are difficult to obtain.

a Department of Clinical Immunology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
b Medical Biotechnology Unit, National Center for Genetic Engineering and Biotechnology, National Science and Technology Development Agency at the Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.

* Corresponding author, E-mail: watchara@chiangmai.ac.th