ScienceAsia 29 (2003): 089-094 |doi: 10.2306/scienceasia1513-1874.2003.29.089

Sequencing and Characterization of
Thai Papaya Ringspol Virus Isolate Type P (PRSYthP)

Gulsirl Charoensllp, Pongsopee Attasart, Mila Juricek, Sakol Panyim and Sunee Kertbundit^*

ABSTRACT: The complete nucleotide sequence of Papaya ringspot virus type p. Thai isolate (PRSVthP) was determined. The viral genome is 10323 nucleotides long and contains an open reading frame encoding a polyprotein of 3343 amino acids. flanked with 5' and 3' non coding regions of 85 and 206 nucleotides, respectively. Among ten putative proteins, PI protein was the most variable (73.9% similarity) when compared to the other full PRSV sequences. while Cl protein was the most conserved protein (99.1% similarity). Sequence similarity among the type P and type W isolates also suggests that the P type arose locally from type W. However, no significant difference between types P and W was discovered that would account for the host specificity.

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Institute of Molecular Biology and Genetics, Mahidol University, Salaya Campus, Nakhon Pathom 73170, Thailand.

* Corresponding author, Email: stskb@mahidol.ac.th

Received 8 May 2002, Accepted 22 Jul 2002