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Research Article

ScienceAsia 27 (2001) : 149-156 |doi: 10.2306/scienceasia1513-1874.2001.27.149

 

A short-term in vitro cultivation of Thai isolate Theileria sp.


Peerapan Tan-ariyaa,*, Chanutree Kerdmaneea,#, Nopporn Sarataphanb and Mathirut Mungthinc


ABSTRACT: The short-term in vitro cultivation of intraerythrocytic stages of Thai isolates of the Theileria parasite was successfully established in this study. Of the four types of culture media used in the experiment, M199 supplemented with 40% fetal bovine serum (FBS) proved to be the best medium. The mean percentage of living parasites cultured in this medium was significantly higher than that in the others, ie, M199+fetal calf serum (FCS), RPMI+FBS and RPMI+FCS (p<0.05). In addition, many developmental stages, ie, match form, ring form, and 2-piroplasm, 3-piroplasm and 4-piroplasm forms, were observed at significant numbers in M199+FBS medium after 48-72 h of cultivation. It was also demonstrated in this study that the blood samples with a high percentage of parasitemia, ie, >4.5%, should not be cultured at cell suspension concentrations higher than 12.5%, whereas those with low percentage of parasitemia, ie, <4.5%, could be cultured at any percentage of cell suspension. On the other hand, our results revealed that culturing at approximately 12.5% cell suspension provided the highest percentage of both multiplied form and living parasites. Production of an optimal cultivation ystem that would lead to the successful continuous in vitro cultivation of intraerythrocytic stages of Theileria parasite remains to be investigated.

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a Department of Microbiology, Faculty of Science, Mahidol University, Rama 6 Road, Bangkok 10400, Thailand.
b Parasitology Section, National Institute of Animal Health, Department of Livestocks Development, Bangkhen, Bangkok 10900, Thailand.
c Department of Parasitology, Phramongkutklao College of Medicine, Rajavithi Road, Bangkok 10400, Thailand.

* Corresponding author, E-mail: scptn@mahidol.ac.th
# Present address:Hematology Section, Somdejprapinklao Hospital, Bangkok 10600, Thailand.

Received 22 Jan 2001, Accepted 3 May 2001