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Research Article

ScienceAsia 27 (2001) : 137-141 |doi: 10.2306/scienceasia1513-1874.2001.27.137

 

Effect of BA and 2iP on Shoot Proliferation and Somaclonal Variation of Gardenia jasminoides Ellis in vitro Culture


Ngarmnij Chuenboonngarma,b, Suvimon Charoonsotea and Sutatip Bhamarapravatia,*


ABSTRACT: Shoot tips of Gardenia jasminoides Ellis were successfully propagated in B5 agar medium with BA (0, 2.5, 5, 7.5 and 10 mg.l-1) and 2iP (0, 2.5, 5, 7.5 and 10 mg.l-1) concentrations. The number of shoots in medium with 10 mg.l-1 BA was 7 times greater than those in medium without BA. The BA induced plantlets had 100% survival rate and normal growth rate ex-vitro. The number of shoots in medium with 7.5 mg.l-1 2iP was 4 times greater than in 2iP free medium. All explants receiving 2iP gave chimeric plants showing different degrees of white leaf tissue. This is the first report of somaclonal variation induced by 2iP in G. jasminoides. Chimeric plants had 70% survival rate and showed slow growth ex-vitro.
Abbreviations: BA-6-Benzylaminopurine, 2iP - 6-(,-dimethylallyamino) purine, B5 - B5 medium, MS - Murashige and Skoog medium

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a Department of Plant Science, Faculty of Science, Mahidol University, Rama VI Road,
Bangkok 10400, Thailand.
b Research Reproductive Biology of Economic Plants, Institute of Science and Technology for Research and Development, Mahidol University Salaya, Nakorn Pathom 73170, Thailand.

* Corresponding author, E-mail: grsbm@mahidol.ac.th

Received 2 May 2000, Accepted 11 May 2001