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Research Article
ScienceAsia 27 (2001) : 021-028 |doi: 10.2306/scienceasia1513-1874.2001.27.021
Isolation and Characterization of a Phenanthrene-Degrading Sphingomonas sp. Strain P2 and Its Ability to
Degrade Fluoranthene and Pyrene via Cometabolism
Nuttapun Supakaa,*, Pairoh Pinphanichakarna, Kobchai Pattaragulwanita,
Suthep Thaniyavarna, Toshio Omorib, and Kanchana Juntongjina
ABSTRACT: A bacterial strain P2 capable of using phenanthrene as a sole source of carbon and energy
was isolated from a lubricant-contaminated soil sample collected from a garage in Prajinburi province,
Thailand and identified by 16S rRNA gene sequence analysis to be in the genus of Sphingomonas. The
Sphingomonas sp. strain P2 rapidly degraded phenanthrene from 100 mgl-1 to undetectable level by
HPLC analysis within 72 h. Besides phenanthrene, this strain was able to degrade a wide variety of
polycyclic aromatic hydrocarbons (PAHs) including naphthalene, acenaphthylene, acenaphthene,
dibenzofuran, fluorene, and anthracene, meanwhile, it was also capable of co-metabolizing high molecular
weight PAHs such as fluoranthene and pyrene in liquid minimum medium supplemented with phenanthrene.
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a Department of Microbiology, Faculty of Science, Chulalongkorn University, Phayathai Road, Pathumwan, Bangkok 10330, Thailand.
b Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan.
* Corresponding author, E-mail: nattapuns@hotmail.com
Received 19 Jul 2000, Accepted 14 Nov 2000
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