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Research Article

ScienceAsia 25 (1999) : 173-177 |doi: 10.2306/scienceasia1513-1874.1999.25.173

 

Effect of Column Length on Forecasted Retention Times and Carbon Numbers in an Isothermal and Temperature-programmed Gas Chromatography

Suwimol Nilratnisakorn, Narumon Jeyashoke and Kanit Krisnangkura*


ABSTRACT: The retention factor (k’) of a fatty acid methyl ester (FAME) in a 30 meter Omegawax 250 capillary column can be accurately predicted by the following equation:

Where n is the carbon number or equivalent carbon number (ECN) and T is the absolute temperature. The four numeric constants of the above equation are not sensitive to column length and they can be employed to calculate the ECN of FAMEs eluted out from the shorter columns of 27, 24 and 21 meters. In addition, these four numeric constants can be employed to forecast the retention times of FAMEs eluted out from the aforementioned columns in the temperature-programmed gas chromatography (TPGC).

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School of Bioresources and Technology, King Mongkut’s University of Technology Thonburi,
Bangkok 10140, Thailand.

* Corresponding author: E-mail: ikamkura@cc.kmutt.ac.th

Received 23 February 1999