ScienceAsia 25 (1999) : 073-075 |doi: 10.2306/scienceasia1513-1874.1999.25.073

Direct Gene Transfer into Skeletal Muscle of Seabass ( Lates calcarifer ) and Black Tiger Prawns ( Penaeus monodon)

Zohrah Haji Sulaiman^*

ABSTRACT: Gene expression after injection of plasmid pCMV-gal into muscle of seabass (Lates calcarifer) and black tiger prawns (Penaeus monodon) was followed for 14 days. It was found that -gal expression was first detected 1 day after injection in seabass and 2 days after injection in prawns. Expression persisted up to 7 and 14 days after injection in seabass and prawns, respectively. Persistence of -gal expression appears to be short-term in seabass, but this is probably due to high variability of gene expression within samples. The relatively low intensity of -gal expression in prawns may suggest specificity of the CMV-1E promoter to vertebrates. Incidence of samples positive for -gal expression in seabass and prawns was 33.3% (7/21) and 20% (6/31), respectively.

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Department of Biology, Faculty of Science, Universiti Brunei Darussalam, Tungku BE 1410,
Negara Brunei Darussalam.

* Corresponding author: E-mail: zohrah@ubd.edu.bn

Received 9 Jan 1999