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Research Article
ScienceAsia 23 (1997): 087-100 |doi: 10.2306/scienceasia1513-1874.1997.23.087
NUCLEOTIDE SEQUENCES OF THE GENES CODING FOR THE MIDDLE SURFACE PROTEIN FROM HEPATITIS B VIRUS GENOMES SUBTYPES adr AND adw ISOLATED IN THAILAND
DUANTHANORM THAWARANANTHA1, KRUAYON BALACHANDRA1, CHUENCHIT BOONCHIRD2, CHEDSADAPORN PITUKSUTEEPONC1, JITTAPORN WATANASEREE3 AND SOMSAK PANTUWATANA4
ABSTRACT: Viral genomes of 8 isolates of hepatitis B virus (HBV) derived from blood specimens were used to sequence of pre-S2 and S regions. Four samples of each subtype adr and adw were used. It was found that there was quite high homogeneity among four samples of the same subtypet that is, 99.6% among samples of adr subtype and 98.7% among samples of adw sub type. Although, the two subtypes showed many nucleotide variationst all of them presented the pattern of wild type DNA. Results showed that the isolated DNA of subtype adw could be classified as genotype B whereas those of subtype adr were classified as genotype C. Concerning the most common amigenic determinant a, no variation as vaccine-induced escape mutant was observed in all eight samplest for examplet at amino acids 126 and 145 of HBsAg.
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1 National Institute of Healtht Department of Medical Sciencest Nonthaburi, 11000.
2 Department of Biotechnology, Faculty of Science, Mahidol University.
3 Government Pharmaceuticals Organization.
4 Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
Received April 14, 1997
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