ScienceAsia 22 (1996): 325-332 |doi: 10.2306/scienceasia1513-1874.1996.22.325

CLONING AND RESTRICTION ENDONUCLEASE ANALYSIS OF THE HEPATITIS B VIRAL GENOME

KRUAVON BALACHANDRA^a, DUANTHANORM THAWARANANTHA^a, CHUENCHIT BOONCHIRD^b, DUANGJAI TECHAYINGPAIBOON^a, CHETSADAPORN PITAKSUTHEEPONG^a, JITTAPORN WATANASEREE^c KASAMA SUPANARANOND^a, AND SOMSAK PANTUWATANA^d

ABSTRACT: The HBV-DNA isolated from Dane particles of three cases of hepatitis carriers was successfully cloned into the BamHI site of the pBR322 plasmids. Three recombinant plasmids with full length HBV-DNA from these three individuals were obtained. The comparison between our cloned HBV-DNAs and four clones reported by others revealed the significant restriction sites of endonucleases in our clones. These restriction sites of endonucleases were shown to be useful in the identification of adr and adw subtypes. All of our clones were also confirmed as adr subtype by the restriction patterns. Moreover, we could find the heterogeneity occurs in the same subtype. However, our clones are very similar to each other in restriction patterns. These results suggested that all of our cloned HBV-DNA might be in the same genotype.

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a National Institute of Health, Department of Medical Sciences, Nonrhaburi 11000
b Department of Biotechnology, Faculty of Science, Mahidol University
c Government Pharmaceutical Organization
d Department of Microbiology, Faculty of Science, Mahidol University, Thailand.

Received July 16,1996