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Research Article

ScienceAsia 22 (1996): 189-200 |doi: 10.2306/scienceasia1513-1874.1996.22.189

 

EFFECTS OF DIFLUBENZURON AGAINST THE LARVAL STAGES OF ANOPHELES (CELLIA) DIRUS AND ANOPHELES (CELLIA) MACULATUS (DIPTERA : ANOPHELINAE)

 

SUPAPORN RATANATHAMa, PHAM XUAN DINHb, E. SUCHART UPATHAMa, NOUWARATN SUKHAPANTHa AND YAOWALUK CHITRAMVONGa

ABSTRACT: The larvicidal effects of Diflubenzuron (a chitin synthesis inhibitor) on the larval stages of four anopheline species, Anopheles dirus species A, An. dirus species B, An. sawadwongporni and An. maculatus form E were studied. The second instar larvae of the four species were the most sensitive instar, with the EC50 value of 0.000340, 0.000349, 0.000498, and 0.00050 mg/I for An. dirus species A An. dirus species B, An. sawadwongporni and An. maculatus form E respectively. The same instar larvae of An. dirus species A and An. dirus species B were more sensitive to Diflubenzuron than those of An. sawadwongporni and An. maculatus form E. Diflubenzuron induced various degrees of morphological abnormalities in all four anopheline species. Under laboratory conditions, the residual effect of Diflubenzuron at the concentrations of EC50 on the same instar larvae of An. dirus species A and An. maculatus form E lasted for 20 to 28 days and 18 to 34 days respectively.

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a Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand.
b Subinstitute of Malariology, Parasitology and Entomology, Ho-Chi-Minh City, Viet Nam.

Received November 1, 1995