 BackResearch Article ScienceAsia 21 (1995): 229-242 |doi: 10.2306/scienceasia1513-1874.1995.21.229
IN VITRO INDUCTION OF POLYPLOIDY IN WHITE MULBERRY (MORUS ALBA VAR. S54) BY COLCHICINE TREATMENT
SARINEE CHAICHAROEN, ARIYA SATRABHANDHU AND MALEEYA KRUATRACHUEABSTRACT: Young leaf explants of Morus alba var.S54 were cultured on the modified Murashige and Skoog (MS) medium supplemented with 0.5 mg/l BA (N6 - Benzyladenine) and 1.0 mg/l NAA (1-Naphthalene acetic acid) for callus initiation ; and 1.0 mg/l BA and 0.5 mg/l NAA for callus proliferation. Active callus tissues were soaked in sterilized aqueous colchicine solution at various concentrations (0.025 to 0.2%) for 3 to 7 days. Then the callus was placed on the medium containing 1.0 mg/l BA and 0.025 mg/l NAA for a shoot regeneration. For shoot growth, the regenerated shoots were subcultured on the medium containing 0.25 mgll BA and 0.025 mg/l NAA. A root formation occurred when culturing an individual shoot on the half-strength modified MS medium containing 0.1 mg/l IBA (Indole-3-butyric acid). Determination of chromosome number was carried out by chromosome counts of squashed root-tip cells. The highest number of tetraploid plants could be obtained from 0.1% colchicine solution with 3 days soak period treatment (47.22%), whereas, other treatments produced lower number of tetraploid plants with the high percentage of mixoploid. The complete plants were successfully grown in pots containing soil. By comparison, the tetraploid plants exhibited slightly slower growth than diploid plants. Other characteristics of the plants were also observed to be differenti the tetraploid plants have bigger stem diameteri greener and thicker leaves than those of diploid plants. Department of Biology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Received April 3, 1995
|
