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Research Article


ScienceAsia 17 (1991): 123-135 |doi: 10.2306/scienceasia1513-1874.1991.17.123

 

PRODUCTION OF SORBITOL AND ETHANOL FROM SUCROSE BY ZYMOMONAS MOBIUS: SUGAR FERMENTATION

 

BOONYARAS SOOKKHEO,a SUREE PHUTRAKULb AND PAWINEE KANASAWUDb

ABSTRACT: Two Zymomonas strains were compared with respect to their sucrose hydrolysing activity, and subsequent sorbitol and ethanol formation. Z. mobilis IFO 13756 has been used to study sugar fermentation since it provided a higher yield of sorbitol whereas the yield of ethanol was nearly the same. The maximum yield of sorbitol was found when Z. mobilis IFO 13756 used 250 g dm-3 sucrose as carbon source in culture medium pH 7.5 with 10% inoculum by fermentation at 30 oC. The conditions for maximum yield of ethanol were fermentation in medium pH 6.5 with 3% inoculum. Addition of some compounds such as xylose, PEC, glucose and ethanol to the culture medium led to a lower yield of both sorbitol and ethanol. A suitable amount of oxygen in the culture medium is expected to have a limiting effect on growth and sorbitol-ethanol formation.

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a Faculty of Science, Khrungthep University, Bangkok, Thailand
b Faculty of Science, Chiang Mai University, Chiang Mai 50002, Thailand.

Received 26 August 1991