ScienceAsia 17 (1991): 051-056 |doi: 10.2306/scienceasia1513-1874.1991.17.051

THE INHIBITORY ACTlVIIT IN 5-LIPOXYGENASE PATHWAY OF HISPIDULIN FROM MILLINGTONIA HORTENSIS LINN. F.

PRIMCHANIEN MOONGKARNDI^a, NUNTAVAN BUNYAPRAPHATSARA,^b VIMOL SRISUKH^c AND HILDEBERT WAGNER^d

ABSTRACT: Hispidulin, a bioactive flavonoid isolated from the Bower of Millingtonia hortensis Linn. E., was tested for anti-phlorgistic effect by observing the inhIbitory activity in 5-lipoxygenase pathway. The test was performed by incubating the hispidulin with 1-¹⁴C-arachidonic add and porcine leukocyte suspension containing 5-lipoxygenase. After the incubation, the 1-¹⁴C-arachidonic add and its merabolite were separated and quantified by RP-HPLC. Hispidulin showed inhibition of 65% at 64 pM.

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a Department of Microbiology b Medidnal Plants Information Center
c Department of Food Chemistry, Faculty of Pharmacy, Mahidol University, Bangkok 10400, Thailand.
d Institute for Pharmaceutical Biology, Munich University, Germany.

Received 27 February 1991