 BackShort Report ScienceAsia 16 (1990): 097-103 |doi: 10.2306/scienceasia1513-1874.1990.16.097
AMINO ACID ANALYSIS OF PROTEIN HYDROLYSATES BY LITHIUM BUFFER SYSTEM
SATHORN SUWANABSTRACT: A chromatographic procedure for the analysis of amino acid hydrolysates using lithium buffers and a dedicated amino acid analyzer is described. When used with an Hitachi amino acid analyzer model 835-50, the modified system permitted separation to near baseline of 18 common amino acids, plus another 6 amino compounds commonly encountered in such assays. Eludon time from injection to arginine was 90 minutes. A major advantage of this system when only one amino acid analyzer is available is the greatly reduced work load compared to the classical switching over between sodium-based and lithium-based systems. The Scientific and Technological Research Equipment Centre, Chulalongkorn University, Chula Soi 62, Phaya-Thai Road, Bangkok 10330, Thailand. Received 23 April 1990
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