ScienceAsia 15 (1989): 157-169 |doi: 10.2306/scienceasia1513-1874.1989.15.157
DETECTION OF TYPE-COMMON AND TYPE-SPECIFIC HERPES SIMPLEX VIRUS DNA SEQUENCES USING RNA PROBES
WASUN CHANTRATITAa, ERIK A. HENCHALb,c AND CHALOBON YOOSOOKa
ABSTRACT: A nucleic acid hybridization assay to identify clinical isolates of herpes simplex
virus (HSV) was developed using in vitro synthesized, radioactively labelled RNA transcripts from virus-specific DNA fragments cloned in transcription vector pGEM3. RNA probes
derived from the HSV-1 BarnHI Q DNA fragment hybridized strongly with HSV-1 and HSV-2 DNA, while RNA derived from HSV-1, EcoRI I, HindIII - PvuII DNA fragment hybridized with HSV-1 DNA only. The results of HSV typing using both common and type-specific probes correlated well with monoclonal antibody typing and restriction endonuclease analysis. Neither probe bound to cytomegalovirus, Epstein-Barr virus, varicella zoster virus, or pseudorabies virus DNA.
a) Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400,
b) Department of Virology, U.S.A. Medical Component, Armed Forces Research Institute
of the Medical Sciences, APO San Francisco 96346-5000.
c) Current address: Department of Virus Diseases, WaIter Reed Army Institute of the
Medical Sciences, WashingtOn, DC 20307-5100, U.S.A.
Received 11 February 1989