ScienceAsia 14 (1988): 219-223 |doi: 10.2306/scienceasia1513-1874.1988.14.219

CELLULOSE SACCHARIFICATION BY CELLULASES OF S. CELLULOPHILUM IN A MEMBRANE REACTOR

CHARURATNA WORANISRAKUL^a, S. KINOSHITA^b AND H. TAGUCHI^b

ABSTRACT: The saccharification of cellulose was investigated in a membrane, reactor using cellulases of S. cellulophilum. The amount of total sugar and glucose produced was used to evaluate the effect of permeation rate on the hydrolysis. It was found that changing the permeation rate within the range of 15.1 to 26.0 ml/hr has little effect on cellulose hydrolysis in a 50 ml reactor during the 48-hour experiment. Eighty to ninety percent of the sugar produced was glucose. Enzyme inactivation was observed during a consecutive six-day experiment.

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a) Department of Civil Engineering, Faculty of Engineering, King Mongkut's Institute of Technology Thonburi.
b) International Center of Cooperative Research in Biotechnology, Faculty of Engineering, Osaka University, Japan.

Received 17 August 1988