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Research Article


ScienceAsia 11 (1985): 079-091 |doi: 10.2306/scienceasia1513-1874.1985.11.079

 

DETECTION OF GLUCOSE BY USING IMMOBILIZED GLUCOSE OXIDASE

 

SUDARAT MANOCHIOPINIJ, CHAMRAS SAPSAMARNWONG AND KULNAREE SIRISALI

ABSTRACT: We present herein a method for analyzing glucose using an immobilized-enzyme membrane. The enzyme membrane is prepared using glutaraldehyde to couple glucose oxidase to a cellulose acetate membrane which is then attached over the teflon membrane of a Clark-type oxygen electrode. The prepared membrane is suitable for use with the Beckman Glucose Analyzer, and an evaluation of its use in this manner yielded the following results. Analytical accuracy averaged 97.86 - 99.58% by the addition method and 96.22 - 98.31% by the dilution method. Coefficients of variation averaged 1.67% for within-run precision and 3.39% for between-run precision. The present method showed good correlation (r = 0.9752) with the Certified Glucose Reagent method, and can reduce analysis costs by a factor of 100.

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Department of Clinical Chemistry, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.

Received 13 May 1985