ScienceAsia 11 (1985): 031-036 |doi: 10.2306/scienceasia1513-1874.1985.11.031

COMPARISON OF POTENTIAL TOXIGENICITY OF FUSARIUM SPP. FROM THAILAND AND NORTH AMERICA

SAMANIYA SUKROONGREUNG,^a H. BRUNO SCHIEFER^b AND J. DREW SMITH^c

ABSTRACT: Three species of Fusarium isolated from samples obtained from Thailand close to the Thai-Kampuchean border. and suspected trichothecenes producing fungi were in vivo compared their ability to produce trichothecenes (T-2. HT-2). The organisms were cultured on rye at 15 to 25^o C for 24 days. The mouldy samples and autoclaved rye control were extracted with ethyl acetate and a part of each extract was cleaned up by thin layer chromatography and used for rabbit skin bioassay. The oily portion of the extract was reduced by hexane-methanol and the methanol fraction was used for animal inoculation and chemical analysis using gas chromatography and mass spectrometry. Each group of 5 mice was intragastrically inoculated with propylene glycol containing the clean-up sample. The results of skin bioassay, chemical analysis and mouse inoculation were agreeable. None of the three species from Thailand were found to produce neither T-2 toxin nor HT-2. In contrast, F. sporotrichioides from North American and American type of culture collection produced copious amounts of them.

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a. Department of Microbiology, Faculty of Medical Technology, Mahidol University, Bangkok 10700, Thailand.
b. Toxiocology Research Centre, University of Saskatchewan, Soskatoon, Saskatchewan S7NOWO, Canada.
c. Canada Agriculture Research Station, Saskatoon Saskatchewan S7N OX2, Canada.

Received 11 September 1984